Lipid, Lipoprotein, and Glucose Metabolism Core

UC MMPC HOME    SELECT A TEST    ORDER A TEST    SEMINAR SPEAKERS    POLICIES    NATIONAL MMPC    UC HOME

Our facility at the UC Metabolic Diseases Institute

Phenotype Test Selector
The tests are broken down according to major divisions of metabolism, irrespective of the core in which they are offered.  Clicking on each division will take you to the appropriate core homepage. Please contact us for test pricing.

Factors Affecting Obesity >

Lipid Metabolism >

Glucose Metabolism >

Hormone Assays >

Cardiovascular Services >

Energy Balance Services >

Glucose Metabolism

 

 

Lipid, Lipoprotein and Glucose Metabolism Core
Test Name Number Keywords: Description:
Euglycemic-hyperinsulinemic clamp C1109 insulin metabolism, glucose metabolism, euglycemia, hyperinsulinemic The euglycemic-hyperinsulinemic clamp is used to investigate insulin action and glucose metabolism in the intact, conscious mouse. The use of different glucose or glucose analog tracers with the option of using dual tracers (3-H or 14-C labeled) allows the determination of tissue-specific glucose uptake in addition to whole body glucose production and disposal.

A chronic indwelling catheter is surgically implanted into the right jugular vein of the mouse. After a minimum of 4 days recovery, the mice are fasted the morning of the experiment. The 120 minute protocol begins with a prime-continuous infusion of glucose tracer and a prime-continuous infusion of insulin, at a rate dependant on the goals and conditions of the experiment. Glucose is infused at a rate that maintains euglycemia and is a measure of insulin sensitivity. Tissues can be collected at the conclusion of the clamp for later assessment of tissue-specific glucose uptake as well as other biochemical assays as desired.
Glucagon concentration C1085 glucagon Concentrations of glucagon in plasma or serum are determined using a multiplexing kit available from Millipore Inc. RIA kits are also available from Millipore for measurement of glucagon.
Glucose concentration C1087 glucose, glucose metabolism, glucose disposal Determinations of glucose in plasma/serum/lymph will be made using a specific colorimetric assay.  Reactions will be run in microtiter plates and analyzed on a plate reader.  Using this method assays can be performed on small (5-25ul) samples of plasma or serum.
Insulin concentration C1089 insulin, proinsulin, lymph, CSF Concentrations of insulin in plasma or serum are determined using a multiplexing kit available from Millipore Inc.  ELISA kits are also available from Millipore for measurement of insulin.
Insulin tolerance test C1072 insulin sensitivity, insulin resistance, glucose disposal, glucose disappearance, glucose metabolism Mice will be fasted for 5 hours.  A fasting blood sample will be removed from the tail vein and insulin (0.5mU/g) will be injected into the abdominal  cavity.  Blood samples will be removed from the tail vein 15, 30, 45 and 60 minutes later.  Samples of plasma obtained during the test will be measured for concentrations of glucose and insulin.
Intraperitoneal glucose tolerance   C1070 glucose tolerance, intraperitoneal glucose tolerance,  insulin secretion, glucose metabolism, glucose disposal Mice will be fasted for 5 hours.  A fasting blood sample will be removed from the tail vein and a concentrated solution of glucose injected into the abdominal cavity of the mice through a needle passed through the abdominal skin.. Blood samples will be removed from the tail vein 15, 30, 60 and 120 minutes later.  Samples of plasma obtained during the test will be measured for concentrations of glucose and insulin.
Oral glucose tolerance   C1071 glucose tolerance, oral glucose tolerance, insulin secretion, glucose metabolism, glucose disposal Mice will be fasted for 5 hours.  A fasting blood sample will be removed from the tail vein and a concentrated solution of glucose infused into the stomach of the mice through a biofeed needle passed through the mouth. Blood samples will be removed from the tail vein 15, 30, 60 and 120 minutes later.  Samples of plasma obtained during the test will be measured for concentrations of glucose and insulin.

 

 


Lipid, Lipoprotein, and Glucose Metabolism Core
Select a Test | Order a Test | Policies | Seminar Speakers | Contact Us
UC MMPC Home | National MMPC | UC Home

Funded by the US Department of Health and Human Services and the National Institutes of Health NIDDK

Center Director | Patrick Tso, Ph.D, 513-558-2151 | Patrick.Tso@uc.edu
Center Administrator | Dana Lee, 558-2748 | Dana.Lee@uc.edu